Job Posting #898757
Research Technician II-III
Krembil Research Institute
$54,522 to $68,172 per annum (Commensurate with experience and consistent with UHN compensation and policy)
37.5 hours per week
Temporary Full-Time (1 year)
University Health Network (UHN) is looking for an experienced professional to fill the key role of Research Technician II or III in our Vision Department.
Transforming lives and communities through excellence in care, discovery and learning.
The University Health Network, where “above all else the needs of patients come first”, encompasses Toronto Rehabilitation Institute, Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre and the Michener Institute of Education at UHN. The breadth of research, the complexity of the cases treated, and the magnitude of its educational enterprise has made UHN a national and international resource for patient care, research and education. With a long tradition of groundbreaking firsts and a purpose of “Transforming lives and communities through excellence in care, discovery and learning”, the University Health Network (UHN), Canada’s largest research teaching hospital, brings together over 16,000 employees, more than 1,200 physicians, 8,000+ students, and many volunteers. UHN is a caring, creative place where amazing people are amazing the world.
University Health Network (UHN) is a research hospital affiliated with the University of Toronto and a member of the Toronto Academic Health Science Network. The scope of research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. Research across UHN's five research institutes spans the full spectrum of diseases and disciplines, including cancer, cardiovascular sciences, transplantation, neural and sensory sciences, musculoskeletal health, rehabilitation sciences, and community and population health. Find out about our purpose, values and principles here.
The Principal Investigator is an Associate Professor at the University of Toronto, and a Senior Scientist in the Donald K. Johnson Eye Institute, located within the Krembil Research Institute at the University Health Network (UHN). Their research laboratory focuses on the use of animal and human stem cell-derived 3D organoids to study and develop treatments for neurodevelopmental, retinal and neurodegenerative disorders. This includes establishing patient-specific induced pluripotent stem (iPS) cells from individuals and use gene editing to generate isogenic models; using a variety of multi-omic approaches to understand disease pathophysiology, and developing therapeutics using gene-based and molecular strategies.
Working under the direction of the Principal Investigator, the responsibilities of the Research Technician II-III include but not limited to maintaining cell lines (cultured and primary), including multipotent and pluripotent stem cell cultures, using standard sterile techniques; generate and maintain 3D organoids using pluripotent stem cell cultures; and generate viral preps for research use. The Research Technician will primarily conduct experiments to support ongoing lab research projects (Western blots, qRT-PCR, immunocytochemistry and immunohistochemistry) and coordinate the collection and organization of patient samples for stem cell derivation. The candidate will also be responsible in lab management, reagent/material and reagent ordering and record-keeping, support the day-to-day operations of the laboratory, assist the research team on daily lab tasks, help execute standard operating procedures and protocols for lab-related activities, and perform other administrative duties.
- At minimum, a Bachelor’s degree in biological sciences or equivalent discipline
- At minimum, three (3) years of experience as a research technician with experience in multipotent and pluripotent stem cell cultures
- Prior experience in standard laboratory protocols for cell culture, Western blot, qRT-PCR, DNA electrophoresis and genotyping, molecular cloning is required
- Prior experience with primary cell isolation from tissue specimens (animals or human), animal models of disease, viral transduction and genetic engineering / gene-editing (e.g., CRISPR/Cas9), and RNA sequencing and bioinformatics is an asset
- Demonstrated interest and ability in continuous learning
- Capacity to work independently with minimal supervision
- Highly-organized and able to prioritize effectively
- Able to communicate and interact with staff, students, colleagues in a clear and professional manner
- Able to work flexible hours on occasion (including weekends or evenings) and travel onsite for research (eg. for stem cell cultures and/or laboratory emergencies)
- Ability to work well under pressure with fast turnaround times, and help to coordinate a rotating shift schedule when necessary
Vaccines (COVID-19 and others) are a requirement of the job unless you have an exemption on a medical ground pursuant to the Ontario Human Rights Code.
If you are interested in making your contribution at UHN, please apply on-line. You will be asked to copy and paste as well as attach your resume and covering letter. You will also be required to complete some initial screening questions.
May 12, 2022 Closing Date: May 26, 2022
For current UHN employees, only those who have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, and possess all the required experience and qualifications should apply.
UHN thanks all applicants, however, only those selected for an interview will be contacted.
UHN is a respectful, caring, and inclusive workplace. We are committed to championing accessibility, diversity and equal opportunity and welcomes all applicants including but not limited to: all religions and ethnicities, LGBTQ2s+, BIPOC, persons with disabilities and all others who may contribute to the further diversification of ideas. Requests for accommodation can be made at any stage of the recruitment process providing the applicant has met the Bona-fide requirements for the open position. Applicants need to make their requirements known when contacted.