Research Technician, Physiology & Pharmacology
University of Calgary
Calgary, AB
The Department of Physiology & Pharmacology in the Cumming School of Medicine invites applications for a Research Technician. This Full-time Fixed Term position is for approximately 1 year (based on length of grant funding), with the possibility of extension.

This position reports to the Principal Investigator.

The Research Technician’s primary purpose is to be responsible for the technical operation and maintenance of the organoid cell culture platform run by the MacNaughton and Hirota labs. This will include generation of organoids and maintenance of cell cultures. The technician will work closely with and receive direction from the platform’s technical director on day-to-day operations of the platform. They will work on a variety of research projects requiring mouse or human primary intestinal epithelial cells.

Some occasional evening or weekend work will be required.

Position Description

Summary of Key Responsibilities (job functions include but are not limited to):

Works with and takes direction from the organoid platform technical director
Responsible for the maintenance of organoid cell cultures to be used in various research projects
Recommends and implements changes to methods, procedures, and practices within the organoid platform
Generate and maintain cultures of primary cells from mouse tissue and human biopsies
Maintain cultures of engineered cells for the preparation of conditioned media for organoid culture
Follow standard operating procedures for the preparation, use and storage of 2D and 3D cultures of primary epithelial cells (organoids) from mouse intestine and human intestinal biopsies
Order supplies required for the organoid platform and maintain platform inventory
Assist in experiments involving these primary cell cultures
Attend and participate in regular lab meetings
Seek to improve techniques and procedures to generate and maintain primary cell cultures
Interact with faculty, students, postdoctoral fellows and other staff in a professional and collegial manner
Responsible for repairs of equipment, ensuring safety compliance
Oversees reliable and safe operation of all laboratory equipment for various users
Keeps laboratory and work area clean and safe; ensures compliance with safety regulations
Establishes and maintains effective, productive relationships with staff, peers, immediate supervisor and senior management, and with the campus community and external partners
Manages own time effectively and ensures immediate reports-to manager is kept informed of activities and progress of work

Qualifications / Requirements:
BSc in the biological or life sciences
Minimum 2 years research experience in a wet lab environment
Experience involving mouse models and mammalian cell culture techniques is essential
Knowledge of theory and practical application of technical procedures and techniques related to cell culture
Experience with managing research finances and accounts
Willingness to work with mice and with human tissue samples
Ability to provide technical support for other team members through advice on technical lab issues
Must be open to learning and development, and be willing to accept new challenges and assignments
Understanding of safety codes and standards related to specialization
Ability to operate and maintain technical equipment related to cell culture
Demonstrated communication, organization, problem solving, and interpersonal skills
Demonstrated knowledge of and compliance with human and animal ethics regulations, including experience with the handling, storage and shipping of human tissues
Intermediate to advanced skills in Microsoft Office Suite (Word, Outlook, Excel, PowerPoint), GraphPad, Adobe Illustrator, internet usage
Knowledge of University core business systems is desirable
Application Deadline: January 22, 2019

We would like to thank all applicants in advance for submitting their resumes. Please note, only those candidates chosen to continue on through the selection process will be contacted.